Stanford Researchers Nab Gene For Narcolepsy in Dogs

Washington, DC - August 5, 1999

After a decade-long search, a Stanford-led team has identified a gene that causes the sleep disorder narcolepsy - a breakthrough that brings a cure for this disabling condition within reach, the scientists say.

The researchers, led by Emmanuel Mignot, MD, PhD, associate professor of psychiatry at Stanford University School of Medicine, used a technique called positional cloning to pinpoint the gene in dogs, one of the few species besides humans known to develop narcolepsy. Mignot predicts the finding will not only help the roughly 135,000 Americans who suffer from narcolepsy, but in time it will shed light on two of the biggest mysteries in sleep research: how and why we sleep.

"This discovery is the exciting culmination of 36 years of research on narcolepsy at Stanford," said William Dement, MD, PhD, director of the Sleep Disorders Research Center at Stanford.

"Narcolepsy is the only known neurological disorder that affects the brain and sleep in such a dramatic way," said Mignot, who is director of Stanford's Center for Narcolepsy. It affects both humans and animals and has both genetic and sporadic forms. Usually beginning in adolescence, narcolepsy's symptoms include extreme daytime sleepiness, sleep paralysis -- a frightening inability to move shortly after awakening or shortly after dozing off -- as well as vivid auditory or visual hallucinations while asleep or while falling asleep.

The most dramatic symptom, however, is sudden episodes of muscle weakness known as cataplexy. The knees may buckle, the neck muscles may go slack, and in extreme cases the person may collapse to the floor completely paralyzed. Loss of muscle tone can last from a few seconds to several minutes. These abrupt attacks can occur at any time but are often triggered by strong emotions such as anger, joy or surprise. It's common for narcoleptics to have such an attack while laughing.

Narcolepsy ruins lives, Mignot said, especially since an average of 14 years pass before the disorder is diagnosed. Because their sleep at night is disrupted, narcoleptics are usually drowsy during the day. Constant sleepiness often impairs performance in school and makes it hard to hold down a job. Not surprisingly, narcoleptics are also accident prone, with about 10 times the rate of auto accidents as the general population. Moreover, since cataplexy often occurs in the presence of friends and can be very embarrassing, narcoleptics may try to suppress their emotions or shun social situations.

The exact prevalence of narcolepsy is uncertain, but surveys in Europe, Japan and the United States suggest about 1 person in 2,000 is affected to some degree.

In the August 6 issue of Cell (*Mignot, E., et al. "The Sleep Disorder Canine Narcolepsy Is Caused by a Mutation in the Hypocretin (Orexin) Receptor 2 Gene." Cell, August 6, 1999.), Mignot and his colleagues report locating two defective versions of the narcolepsy gene, one in Doberman pinschers, the other in Labrador retrievers. The gene, known as hypocretin receptor 2, codes for a protein that juts out from the surface of brain cells and that functions as an antenna, allowing the cell to receive messages - transmitted via small molecules called hypocretins - from other cells. The defective versions of the gene encode proteins that cannot recognize these messages, in effect cutting the cell off from essential directives, including perhaps messages that promote wakefulness.

The researchers know that the same gene exists in humans, and they plan to search for defective versions in narcoleptic individuals, Mignot said. (For an update on this topic, see the August 2000 announcement concerning this gene in humans.)

"You are happy when you make a discovery, but you are really, really happy when you make a discovery with therapeutic possibilities," Mignot said. He noted that current treatments for narcolepsy - doses of stimulants to maintain alertness during the day - alleviate some symptoms but do not influence the underlying brain defect. With the gene in hand, however, it should be possible to design a drug that can compensate for the failure of the hypocretin system, Mignot said. He expects great interest in academia and among pharmaceutical companies to create drugs that can stimulate or block the hypocretin receptor. Such drugs might even be modified versions of the hypocretins themselves, he added.

The discovery also might help drug designers produce sleeping pills that more closely mimic natural brain chemistry, he said.

Mignot and his colleagues began hunting for the narcolepsy gene in dogs more than a decade ago. They chose dogs in part because the animals get narcolepsy, but also because the genetics of the canine disorder are simple in some breeds. In Dobermans and Labradors, narcolepsy is caused by a single gene, and the trait is recessive, meaning that an animal must inherit a defective copy of the gene from both parents to be affected.

In people, the situation is much more complicated. While the disorder occasionally runs in families, most narcoleptics have no affected relatives. Mignot said the inheritance of human narcolepsy resembles diseases like heart disease and diabetes, in which several genes contribute to susceptibility but the disorder usually will not develop without a specific environmental trigger.

Finding the gene was particularly challenging, Mignot said, because, unlike the situation with well-studied animals like mice and humans, little was known about the organization of the dog's genes. The team gradually narrowed their search, refining their map of the dog's genes along the way, until they pointed to a single gene on the dog's 12th chromosome.

By implicating hypocretins in sleep regulation, the work also opens up new areas of research for sleep scientists, according to Mignot. These molecules were known to control eating, but their role in sleep is unexpected. "Hypocretins are going to be a major player in the understanding of sleep," he said.

This discovery would not have been possible without the 10 years of research support for the dog colony from the National Institute of Neurological Disorders and Stroke at the National Institutes of Health, Mignot said.

"The discovery of the narcolepsy gene holds the promise of allowing development of specific therapies to address the disorder, perhaps even obviating the need for controlled drugs as treatment," said Charlotte McCutchen, MD, program officer at the National Institute of Neurological Disorders and Stroke. "With the earlier demonstration that narcolepsy is the result of a neurodegenerative process, the discovery of its gene may lead to a better understanding of many of the neurodegenerative disorders. This discovery also may lead to an increase in our understanding of other sleep disorders and of the role of sleep itself in our lives," she said.

Additional Statement from the National Institutes of Health

Statement from Gerald D. Fischbach, M.D., Director, National Institute of Neurological Disorders and Stroke, and Claude Lenfant, M.D., Director, National Heart, Lung, & Blood Institute

The discovery of the gene for narcolepsy in dogs by Dr. Emmanuel Mignot and his group at Stanford University School of Medicine has particular importance and significance for the fields of sleep and neurology and for patients with sleep disorders.

Dr. Mignot's study, funded by the National Institutes of Health and published in the August 6 issue of Cell, opens the door to identification of the narcolepsy gene in humans and to development of new treatment approaches and possibly a cure for this disabling sleep disorder.

As important, this is the first time that the recently discovered family of brain neuropeptides known as hypocretins has been identified as a significant player in regulating sleep. This represents a giant step toward understanding the molecular basis of sleep, a major aim of sleep research at the National Institutes of Health.

This discovery not only links a gene to a disease, but paves the way to an improved understanding of the fundamental nature of sleep and its contribution to human health and well-being and ultimately should help us treat and possibly prevent many other sleep disorders as well.